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Regular Member Meeting 2017 09 12: Difference between revisions

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=== Project CRISPR -Enrique ===
=== Project CRISPR -Enrique ===
We repeated the protocol carefully with Sophia doing the plating and protocol and good temperature control during the process. The control worked OK (bacteria still survive if there is no antibiotic) but the CRISPR sample did not. This is the fourth failure and we're going to contact the kit vendor for advice and maybe some assistance. The proteins or DNA may be degrading in storage because we don't have a constant-temperature -20C freezer, for example. Regardless, we're planning to press ahead with a Saturady workshop so people can learn about CRISPR and get a hands-on feeling for what the process is like. [email protected]
We repeated the protocol carefully with Sophia doing the plating and protocol and good temperature control during the process. But it didn't work, again. I suspect our freezer may not be good enough for long-term storage of the protein and DNA ingredients. I've written the vendor to ask for help because we've spent over $500 in both HacDC money and donated supplies and equipment so far and it hasn't worked once. Regardless, we're planning to press ahead with a Saturday workshop so people can learn about CRISPR and get a hands-on feeling for what the process is like. Nancy and Sophia are working on a video. [email protected]


=== Project SpaceBlimp -Enrique ===
=== Project SpaceBlimp -Enrique ===

Revision as of 01:13, 8 September 2017


Time and Location

Date
Called to order at xxxx pm by xxxx
Members present:
Members remote:
Others present:
Quorum
Quorum met?

Approval of Previous Meeting's Minutes

Recognition

New Members

Old Business

New Business

Project Awesome Reports

Project SUPPLIES - Mirage

Project EXPANSION - Mirage

Project HAMRADIO - Jeff

Project Electron Microscope (SEM) - Enrique

I am not working on this; too many other things. Julia et al are working on providing a digital interface to the new vacuum gauge with an opto-isolator.

Project Optical Table Robot (OTR) -Julia

Project CRISPR -Enrique

We repeated the protocol carefully with Sophia doing the plating and protocol and good temperature control during the process. But it didn't work, again. I suspect our freezer may not be good enough for long-term storage of the protein and DNA ingredients. I've written the vendor to ask for help because we've spent over $500 in both HacDC money and donated supplies and equipment so far and it hasn't worked once. Regardless, we're planning to press ahead with a Saturday workshop so people can learn about CRISPR and get a hands-on feeling for what the process is like. Nancy and Sophia are working on a video. [email protected]

Project SpaceBlimp -Enrique

We aborted the launch of Spaceblimp 7 during the solar eclipse. Long story short we were not ready and driving 8 hours down to the SC border on a weekday was a high bar for a group of volunteers. We should do Spaceblimp 7 more locally just as Spaceblimp 6 and take our time testing things and making an interesting payload. I now have a cylinder of Helium at home that we're paying $0.20/day to rent, so we're actively losing money not doing anything. But the tracker(s) are still broken or untested, for example. Contact [email protected] for more information.

Project Media Outreach - Matt

Project IT Infrastructure - Tom

Member Reports

Director Reports (Governance related stuff)

President's Report

Vice President's Report

Treasurer's Report

Secretary's Report

Director-at-Large Reports

Mike

Matt

Adjournment

Adjourned by at xxx by xxx