Physiological Saline Testing: Difference between revisions
From HacDC Wiki
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====Overview==== | ====Overview==== | ||
==== Example Formulation==== | ==== Example Formulation Materials (1 liter) ==== | ||
* sodium chloride | * sodium chloride (8 g) | ||
* potassium chloride | * potassium chloride (0.2 g) | ||
* sodium phosphate, dibasic | * sodium phosphate, dibasic (1.15 g) | ||
* potassium phosphate, monobasic | * potassium phosphate, monobasic (0.2 g) | ||
* distilled or double distilled water (1 L) | |||
There are other formulations available if, for example, we want to do a batch of 10X PBS to store and dilute as needed. | |||
=== Additional Materials Required === | |||
* a good balance to weigh the materials on | |||
* glassware for measurement and storage | |||
* pH strips are do-able, but a pH meter would be preferred | |||
=== Storage Requirements === | |||
If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration). | |||
=== Possible Vendors === | |||
If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees. | |||
* Sigma-Aldrich (http://www.sigmaaldrich.com/united-states.html) | |||
* Invitrogen (http://products.invitrogen.com/ivgn/product/18912014) | |||
* Amresco (http://www.amresco-inc.com/) | |||
[[Category:GrindDC]] | [[Category:GrindDC]] | ||
Revision as of 00:55, 15 November 2012
Overview
This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.
Solutions
Phosphate Buffered Saline (PBS)
Overview
Example Formulation Materials (1 liter)
- sodium chloride (8 g)
- potassium chloride (0.2 g)
- sodium phosphate, dibasic (1.15 g)
- potassium phosphate, monobasic (0.2 g)
- distilled or double distilled water (1 L)
There are other formulations available if, for example, we want to do a batch of 10X PBS to store and dilute as needed.
Additional Materials Required
- a good balance to weigh the materials on
- glassware for measurement and storage
- pH strips are do-able, but a pH meter would be preferred
Storage Requirements
If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).
Possible Vendors
If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.
- Sigma-Aldrich (http://www.sigmaaldrich.com/united-states.html)
- Invitrogen (http://products.invitrogen.com/ivgn/product/18912014)
- Amresco (http://www.amresco-inc.com/)